wst 8 assay  (MedChemExpress)

Journal: Frontiers in Oral Health

Article Title: Novel small molecule targeting PgQC reduces Porphyromonas gingivalis virulence

doi: 10.3389/froh.2026.1716188

Figure Lengend Snippet: S-0636 exhibits a low potential of cytotoxicity in human cell lines. Human liver cells (HepG2, black bars), human embryonic kidney cells (HEK293, grey bars), human gingival keratinocytes (hTERT-TIGK, dark grey bars), and human gingival fibroblasts (hTERT-TIGF, light gray bars) were seeded and cultivated as described above. Once the cells reached ∼80% confluency, the medium was replaced with fresh medium containing various concentrations of S-0636, followed by an additional 24-hour incubation under the same conditions. The WST-8 assay determined the viability of cells. Cells treated with 0.1% Triton X-100 served as a negative control), while cells treated with the S-0636 compound diluent served as a positive control. Cell viability was quantified by measuring the absorbance of WST-8 formazan at 450 nM using a Tecan Sunrise microplate reader. All experiments were performed in at least three independent biological replicates.

Article Snippet: After treatment, WST-8 reagent (MCE®, MedChem Express) was added at a 1:10 dilution and incubated for 2–3 h at 37 °C.

Techniques: Incubation, Negative Control, Positive Control